An international team led by researchers at the Centre for Palaeogenetics has sequenced DNA recovered from mammoth remains that are up to 1.2 million years old. The analyses show that the Columbian mammoth that inhabited North America during the last ice age was a hybrid between the woolly mammoth and a previously unknown genetic lineage of mammoth. In addition, the study provides new insights into when and how fast mammoths became adapted to cold climate.
“This DNA is incredibly old. The samples are a thousand times older than Viking remains, and even pre-date the existence of humans and Neanderthals”, says senior author Love Dalén, a Professor of evolutionary genetics at the Centre for Palaeogenetics, jointly funded by The Swedish Museum of Natural History and Stockholm University.
Small fragments of DNA
Around one million years ago there were no woolly or Columbian mammoths, as they had not yet evolved. This was the time of their predecessor, the ancient steppe mammoth. Researchers have now managed to analyse the genomes from three ancient mammoths, using DNA recovered from mammoth teeth that had been buried for 0.7-1.2 million years in the Siberian permafrost.
“This is the first time that DNA has been sequenced and authenticated from million-year-old specimens, and extracting the DNA from the samples was challenging,” says Love Dalén.
An unexpected origin
Analyses of the genomes showed that the oldest specimen, which was approximately 1.2 million years old, belonged to a previously unknown genetic lineage of mammoth. The researchers refer to this as the Krestovka mammoth, based on the locality where it was found. The results show that the Krestovka mammoth diverged from other Siberian mammoths more than two million years ago.
“This came as a complete surprise to us. All previous studies have indicated that there was only one species of mammoth in Siberia at that point in time, called the steppe mammoth. But our DNA analyses now show that there were two different genetic lineages, which we here refer to as the Adycha mammoth and the Krestovka mammoth. We can’t say for sure yet, but we think these may represent two different species”, says the study’s lead author Tom van der Valk.
Columbian mammoth a hybrid
The researchers also suggest that it was mammoths that belonged to the Krestovka lineage that colonised North America some 1.5 million years ago. In addition, the analyses show that the Columbian mammoth that inhabited North America during the last ice age, was a hybrid. Roughly half of its genome came from the Krestovka lineage and the other half from the woolly mammoth.
“This is an important discovery. It appears that the Columbian mammoth, one of the most iconic Ice Age species of North America, evolved through a hybridisation that took place approximately 420 thousand years ago”, says co-lead author Patrícia Pečnerová.
Evolution and adaptation
The researchers could now compare the genome from mammoths during a span of one million years. This made it possible to investigate how mammoths became adapted to a life in cold environments and to what extent these adaptations evolved during the speciation process. The analyses showed that gene variants associated with life in the Arctic, such as hair growth, thermoregulation, fat deposits, cold tolerance and circadian rhythms, were already present in the million-year-old mammoth, long before the origin of the woolly mammoth. These results indicate that most adaptations in the mammoth lineage happened slowly and gradually over time.
“To be able to trace genetic changes across a speciation event is unique. Our analyses show that most cold adaptations were present already in the ancestor of the woolly mammoth, and we find no evidence that natural selection was faster during the speciation process”, says co-lead author David Díez-del-Molino.
The new results open the door for a broad array of future studies on other species. About one million years ago was a period when many species expanded across the globe. This was also a time period of major changes in climate and sea levels, as well as the last time that Earth’s magnetic poles changed places. Because of this, the researchers think that genetic analyses on this time scale have great potential to explore a wide range of scientific questions.
“One of the big questions now is how far back in time we can go. We haven’t reached the limit yet. An educated guess would be that we could recover DNA that is two million years old, and possibly go even as far back as 2.6 million. Before that, there was no permafrost where ancient DNA could have been preserved”, says Anders Götherström, a professor in molecular archaeology and joint research leader at the Centre for Palaeogenetics.